Browsing by Author "Akomolafe, R.O."
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Item Assessment of the toxicity and biochemical effects of detergent processed cassava on renal function of Wistar rats(ScienceDirect, 2020) Oghobase, G.E; Aladesanmi, O.T; Akomolafe, R.O.; Akano, O.P.; Olukiran, O.S; Eimunjeze, M.HFufu is a major component of food in West Africa. Local cassava farmers use detergent to ferment cassava root tuber during processing into fufu. Studies have established the hazardous health effects of detergent in humans. This study was carried out to determine the median lethal toxicity of detergent-processed cassava, and assessed its effects on the renal function of male Wistar rats. Fufu with different concentrations of detergent (250, 500 and 750 g/l) were given as food twice daily to the rats for the acute toxicity study. The sub-chronic test lasted for four weeks, during which the fufu samples prepared with 10, 20, 30, 40 and 50 g/l of detergent, were separately given to five groups of five rats, respectively. The results of the acute toxicity test showed increase in mortality with corresponding increase in detergent concentration. Plasma and urine creatinine and urea concentrations of all the detergent-treated rats were significantly higher than that of the negative and positive controls. Urinalysis showed significant presence of glucose and ketone bodies, as well as, blood and protein. Renal photomicrographs showed deranged cortical structure with diffuse loss of tubules, inflammatory cells infiltrate in the interstitium. The study concluded that detergent-processed cassava has adverse effects on the structure and function of the kidneys of rats.Item Effects of Methanol Extract of Senna Podocarpa leaf on the Reproductive Parameters of Male Wistar Rats Following lead Acetate Toxicity(Reproductive System and Sexual Disorders: Current Research, 2020) Akinsomisoye, O.S; Akomolafe, R.O.; Abatan, J.A.; Akano, O.P.; Odukoya, S.O.A; Akintayo, C.OBackground: Lead and its compounds have been implicated to be reprotoxic by inducing testicular damage and causing hormonal imbalance. Senna podocarpa has antimicrobial, anti-inflammatory and wound healing potentials. This study investigated the effects of the methanol extract of Senna podocarpa leaf (MESP) on the reproductive activities of male Wistar rats following the induction of reproductive toxicity using lead acetate. Methods: Thirty male rats with body weights between 170g - 200g (10 - 12 weeks) were used for the study. The rats were divided into 6 equal groups as follows; Group 1 rats received 1 ml/kg of Tween 80 only while group 2 rats received 15 mg/kg lead acetate only. Rats in group 3 received 15 mg/kg lead acetate and 500mg/kg of MESP concurrently while rats in group 4 received 15 mg/kg lead acetate and 1000mg/kg of MESP concurrently. Groups 5 and 6 rats received only 500 mg/kg and1000 mg/kg of MESP respectively. Lead acetate was administered intraperitoneally while MESP and Tween 80 were administered orally. All treatments were for 14 days. Results: It was observed from the results that administration of lead acetate for 14 days induced male reproductive toxicity which was evidenced by testicular and epididymal degeneration, significant decrease in sperm parameters, reproductive hormone and antioxidant level. However, concomitant administration of MESP especially at 1000 mg/kg ameliorated the toxic effect of lead acetate as shown by improved testicular and epididymal histology as well as increased sperm parameters. Conclusion: Our findings suggest that MESP may be used as herbal remedy in cases of reproductive toxicity caused by lead acetate.