Browsing by Author "Alao, Olaoluwa Kehinde"
Now showing 1 - 3 of 3
Results Per Page
Sort Options
Item Amylase production by Streptomyces species and its application in orange juice clarification(Trakya University Journal of Natural Sciences, 2026-01-13) Odjoji, Elizabeth Aghogho; Fasiku, Samuel Adedayo; Alao, Olaoluwa Kehinde; Salawu, Kehinde Olamide; Dada, Micheal Tunde; Odeniyi, Olubusola Ayoola; Wakil, Sherifah MonilolaAmylases are enzymes that break down starch and help clarify fruit juices. This study focused on screening amylolytic Streptomyces spp. isolated from soil samples for their potential for amylase production and fruit juice clarification. Select organisms produced amylase, which was assayed by measuring the reducing sugar content of the fermentation medium. The effects of pH, carbon and nitrogen source, as well as agitation and incubation periods, were evaluated to optimize amylase synthesis. A total of 22 species were isolated, with five—FE4, ELI1, FL2, MS2, and MS5—demonstrating high amylase production ability, which occurred at a pH ranging from slightly acidic to slightly alkaline. Cassava peels supported optimal amylase production in Streptomyces spp. A4 (0.834), ELI1 (0.910), and FE4 (0.814 U/mL). The maximum yield of 0.930 U/mL was observed with ELI1 when urea was used as the nitrogen source, at an agitation speed of 100–150 rpm, and peaking on the fourth day of fermentation. It was identified as S. griseoflavus ELI_1 using 16S rRNA gene sequencing and submitted to the GenBank with accession number OQ930232. The amylase produced by it was partially purified, markedly increasing its specific activity from 1.50 to 4.56 U/mL. Its ability to clarify orange juice was tested; the turbidity reduced significantly by 16.8% after amylase treatment (p <0.05). Amylolytic Streptomyces spp. were isolated from soil samples, and their amylase yield was optimized. The Amylase of S. griseoflavus ELI_1 could optimally clarify orange juice.Item Degradation of lignocellulosic substrates by Pleurotus ostreatus and Lentinus squarrosulus(Conscientia Beam, 2023-05-08) Fasiku, Samuel Adedayo; Wakil, Sherifah Monilola; Alao, Olaoluwa KehindeLignocellulosic substrates are wastes in the environment whose reducing sugars are not readily available for use. Biological pretreatment is the use of microorganisms and/or their metabolites to break down substrates to obtain simple sugars which is also cheap compared with other pretreatment techniques. This work is aimed at degrading lignocellulosic substrates with higher mushrooms to obtain simple sugars that could be used as raw materials for other industrial processes. The two mushrooms [Pleurotus ostreatus (PO) and Lentinus squarrosulus (LS)] with the ability to produce cellulase, xylanase, and lignase were used for degradation of lignocellulosic substrates [groundnut shell (GS), maize cob (MC), maize straw (MS), rice straw (RS) and sugarcane bagasse (SB)]. The residual extractives, cellulose, hemicellulose, lignin, and reducing sugar contents were determined every 7 days. Least extractives (1.12 %), hemicellulose (15.09 %), lignin (17.60 %), and cellulose (5.60 %) were recorded in PO-degraded MS, POLS-degraded GS, LS-degraded GS, and PO-degraded MS at 28, 35, 49 and 42 days of degradation, respectively. The highest reducing sugar contents (mg/g) obtained in GS (11.83), MS (27.03), SB (28.70), and RS (37.96) were recorded when degraded by PO for 49, 14, 7, and 49 days, respectively while that of MC (13.32) was recorded when degraded by LS for 42 days. Reducing sugar obtained was higher from sole degradation with PO compared with LS and POLS. Degraded MS, RS, and SB had better yield of reducing sugar than GS and MC. The amount of reducing sugar released varied with substrates, organisms, and degradation time.Item Screening For Lignocellulolytic Enzymes-producing White Rot Fungi(Asian Journal of Research in Botany, 2023-04-08) Fasiku, Samuel Adedayo; Wakil, Sherifah Monilola; Alao, Olaoluwa KehindeThe three major parts of lignocellulose are cellulose, hemicellulose and lignin which can be broken down by cellulase, xylanase and laccase, respectively, thereby making the reducing sugar in lignocellulose available for industrial processes. This work aimed to screen for white-rot fungi with the potential of producing cellulase, xylanase and laccase which are vital in breaking lignocellulosic substrates. Some white rot fungi were screened for their abilities to produce cellulase, xylanase and laccase on potato dextrose agar supplemented separately with 1% carboxyl methyl cellulose (CMC), 1% of xylan, and 0.1% of 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid), respectively and enzymes relative activity were determined. The highest average relative activity of cellulase (1.07 ± 0.073) was recorded with Lentinus squarrosulus while the same average relative xylanase activity (1.13) was produced by both Lentinus squarrosulus and Pleurotus ostreatus. Pleurotus tuber-regium did not produce cellulase and xylanase. The highest average relative laccase activity (1.43 ± 0.286) was produced by Pleurotus tuber-regium followed by Pleurotus ostreatus (1.24 ± 0.162) and the least was by Lentinus squarrosulus (1.12 ± 0.134). Pleurotus ostreatus and Lentinus squarrosulus produced cellulase, xylanase and laccase which are important in breaking down lignocellulose. Pleurotus ostreatus and Lentinus squarrosulus could be employed to break down lignocellulose.