Browsing by Author "Ojo-Omoniyi, Olusola"
Now showing 1 - 2 of 2
Results Per Page
Sort Options
Item ENHANCEMENT OF SOURDOUGH BREAD USING PROBIOTIC LACTIC ACID BACTERIA ISOLATED FROM CORN STEEP LIQUOR(Journal of Faculty of Food Engineering, Ştefan cel Mare University of Suceava, Romania, 2025-02-27) Fashogbon, Racheal; Popoola, Bukola; Samson, Oyindamola; Akinwunmi, Ismail; Ojo-Omoniyi, OlusolaFermented Zea mays (OGI), an edible paste made from fermented grains specific to the region, is an integral part of the human diet. Corn steeping liquor (CSL) is an important by-product obtained from the wet milling of Zea mays. The present study was conducted to investigate the probiotic characteristics and in vitro antibacterial activity of lactic acid bacteria (LAB) obtained from CSL, as well as their impact on the quality of sourdough bread. A total of 46 LAB were isolated and evaluated for their probiotic potential. The probiotic potential of LAB was determined by evaluating their hemolytic capacity, bile salt resistance, phenol tolerance, antimicrobial and antioxidant activities. The lactic acid bacteria with the most promising probiotic potential were molecularly characterized and used for the production of sourdough bread (SDB). Conventional microbiological methods identified Lactobacillus plantarum (21%), Lactobacillus fermenti, and Leuconostoc mesenteroides (17%) as the predominant LAB species. Twelve LAB showed signs of gamma hemolysis. It is noteworthy that isolate CSL23 showed the highest bile tolerance, while isolates CSL15, CSL23, and CSL6 demonstrated the highest phenol tolerance. Isolates CSL15, CSL23, and CSL29 showed significant inhibition zones of 15.00 ± 0.18 mm, 20.5 ± 0.26 mm, and 22.60 ± 0.31 mm against four tested pathogens, in addition to effective antioxidant scavenging activity. Molecular identification revealed that LAB isolates CSL15, CSL23, and CSL29 were Lactiplantibacillus plantarum ROF4, Lactiplantibacillus plantarum ROF5, and Lactiplantibacillus plantarum ROF6, respectively. This finding indicates that all LAB strains belong to the same genus. Therefore, it is evident that the current study demonstrated that Lactiplantibacillus plantarum strains isolated from CSL have the potential to serve as promising probiotic starter cultures that could be used in the production of sourdough bread with improved functional properties.Item MOLECULAR AND BIOCHEMICAL CHARACTERIZATION OF AUTOCHTHONOUS CELLULOLYTIC MICROORGANISMS FROM THREE LANDFILL SITES IN LAGOS, SOUTHWEST NIGERIA(Journal of Faculty of Food Engineering, Ştefan cel Mare University of Suceava, Romania, 2024-04-03) Ojo-Omoniyi, Olusola; Buraimoh, Maria; Jolaoluwa, Grace; Akindusoye, Opeyemi; Fashogbon, RachealThis study was conducted to evaluate and identify native cellulolytic microorganisms to tropical sanitary landfill sites in Lagos, Southwest Nigeria as well as to provide insights into the potential of autochthonous microorganisms in solid waste management. Soil and leachate samples were obtained simultaneously (0 – 30 cm depth) at the same time from three waste dumpsites in Lagos, using aseptic procedures. Thereafter, composite soil samples and leachates were generated independently and transported to the laboratory for analysis. Microorganisms were isolated from both soil and leachate samples using the serial dilution technique on sterile nutrient agar (NA) and potato dextrose agar (PDA). Subsequently, cellulase-producing microbial species were identified using conventional and standard microbiological techniques as well as by cultivation on Starch-casein-agar. Pure cultures of isolates were inoculated on sterile filter paper placed on Starch - casein agar plate. Isolates were selected based on their metabolic capabilities to utilize the filter paper for growth. Screening for utilization of aromatic acids was carried out in 250 mL conical flasks containing composition: minimal agar medium (pH 7.2), 1.0 g/L aromatic acids (vanillic), 1.0 mL trace elements, phosphate buffer and Bromothymol blue as pH indicator. The DNA of some selected isolates with cellulolytic activity were extracted and sequenced using 16S rRNA sequencing, ITS, and bioinformatics tools. Consequently, among the bacterial species, Bacillus sp. had the highest cellulose degradative ability and was the most prevalent (50%) in occurrence among bacterial species while Aspergillus sp. emerged as the most commonly occurring fungal isolate (35.7%). Data of selected sequenced cellulolytic isolates were deposited at NCBI GeneBank with Accession numbers: KP843680.1 (Vibrio tubiashii), MK748310.1(Aspergillus aculeatinus), LC496490.1 (Aspergillus aculeatus), CP029751.1 (Staphylococcus aureus) and JX144699.1 (Bacillus mycoides). Environmental surveillance of these microorganisms with microbial synergistic capabilities, could transform solid waste management into a highly efficient biotechnological process that facilitates volume reduction, waste recycling and Bioenergy production.