Browsing by Author "Popoola, Bukola Margaret"
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Item Biodegradable Waste(Intechopen, 2023-02-22) Popoola, Bukola MargaretBiodegradable wastes are waste materials easily degraded or broken down naturally by factors such as biotic (bacteria, fungi, plants, animals, etc.) and abiotic (pH, temperature, oxygen, humidity, etc.). This process enables complex substances to be broken down into simpler organic compounds which subsequently fade into the soil. This is a natural process that could be prolonged or rapid and poses little risks to the environment. These waste materials could be termed green waste; including food waste, paper waste, and biodegradable plastics such are found in municipal solid waste. Other examples of biodegradable wastes include sewage, manure, sewage sludge, human waste, waste from various slaughterhouses, hospital waste, dead animals, and plants. Biodegradable waste could be said to be recyclable or reused; furthermore, bio-waste recycling may also directly contribute to climate protection. They are generally known as useful waste. Recycling is one of the current waste management strategies having great benefits for the environment.Item Biodegradative Capability of Molecularly Identified Pseudomonas fluorescens Strains on Palm Oil-Polluted Soil(LYAS, 2026-05-24) Popoola, Bukola Margaret; Afelumo, Ayooluwa; Samson, Oyindamola JohnOil palm (Elaeis guineensis) is an important source of edible vegetable oil derived from the fruit mesocarp; however, oil extraction commonly generates wastewater and residues that may be discharged into the environment without adequate treatment. Because microorganisms can degrade organic contaminants in palm oil mill effluent and oil-contaminated matrices, this study aimed to evaluate the potential of Pseudomonas fluorescens for remediating palm oil-contaminated soil. Palm oil and P. fluorescens isolates were obtained from Fiditi Oil Mill and Ajayi Crowther University, respectively, in Oyo State, Nigeria. Uncontaminated soil, oil mill soil, and purposefully contaminated soil were used as experimental control samples, while four purposefully contaminated soil samples and four oil mill soil samples were treated with P. fluorescens under varying soil sterilization and tillage conditions. The isolate was identified using 16S rRNA gene sequence analysis, and a 12-week field bioremediation trial was conducted to assess microbial load and residual oil content. The 16S rRNA sequencing confirmed the isolate as P. fluorescens MN607218.1. Residual oil in tilled sterile purposefully contaminated soil decreased from 0.13 g on day 0 to 0.10 g by week 12, whereas the corresponding non-sterile soil showed a greater reduction to 0.07 g by week 12. These findings indicate that P. fluorescens can effectively degrade palm oil in contaminated soil, particularly under non-sterile conditions, and may serve as a promising microbial agent for environmental cleanup of palm oil-contaminated sites.Item Fatty Acid Methyl Ester Analysis of Olive Oil Degraded by Candida parapsilosis(Longdom, 2020-02-13) Popoola, Bukola Margaret; Onilude, Anthony AbiodunFatty acids are the major components of lipids; and the physical, chemical and physiological properties of a lipid class depend primarily on its fatty acid composition. Gas chromatography method can be used for the identification of microbiological degraded fatty acids in vegetable oils as methyl ester. The use of GC for FAME Quantification determination with flame ionization detectors has been effectively used for some time. It is highly sensitive, high accuracy and high reproducibility. It has advantages over Mass Spectrometry (MS) when analyzing n-6 and n-3 fatty acid metabolism as it is able to distinguish between structurally similar fatty acids as it uses retention time for labeling as opposed to atomic mass. MS is able to identify fatty acids within a sample but unable to distinguish double bond positions in stereoisomers and therefore unable to tell certain fatty acids apart. Although GC/MS and other technical methods have been developed to quantitate C8-C26 chain-length FAs, the GC analysis of FAs with FID remains the most frequently used method (Jumat et al., 2006). This study monitored the breakdown of olive oil by the lipase of Candida parapsilosis which was grown in two different mineral salt medium, one consisting of (g/L) of KH2PO4, 7.584; K2HPO4-, 0.80; MgSO4 .7H2O, 0.80; CaCl2, 0.16; (NH4)2NO3, 0.80; FeSO4¬, 0.16; and olive oil 2%, PH maintained at 7.0. The second one is also a modification of the first medium consisting (g/L) of KH2PO4, 7.584; K2¬HPO4¬, 0.80; MnSO4.4H2O,0.80; NaCl, 0.16; (NH4)2NO3, 0.80; Fe2(SO4)3, 0.08; and olive oil, 2%, pH maintained at 7.0 was studied over 25 days. The fatty acid profiles of the oils and methyl esters were determined by chromatography analyzer. The olive oil was analyzed for the fatty acids commonly present in it which are Myristic, Palmitic, Stearic, Oleic, Linoleic, Linolenic, Behenic and Lignoceric, having specific carbon number and their values in approximate percentage C14:0 (0.4), C16:0 (14.0), C18:0 (5.5), C18:1 (76.4), C18:2 (3.4), C18:3 (0.1), C22:0 (0.1) and C24:0 (0.1) respectively. Of all the fatty acid, oleic acid percentage is high in olive oil containing considerable amount of 76.4%. The lipase of Candida parapsilosis reduced the fatty acid by 6.7% after 20 days in the media used. Hence the lipase from Candida parapsilosis had potential for degradation of fatty waste, a potential microbial isolate to be used for bioremediation processes.Item Impact of Rhizobium spp. on nodulation of cultivated (Vigna unguiculata) cowpea and soil fertility in southwestern Nigeria(Springer Nature, 2026-01-12) Popoola, Bukola Margaret; Oyatokun, Olukayode Stephen; Ezeoma, Chinenyenwa Mercy; Samson, Oyindamola JohnCowpea, an essential legume in the semi-arid regions of Sub-Saharan Africa, often experiences low yields due to poor cultivars, inadequate management practices, and limited agricultural inputs. This study evaluated the impact of Rhizobium inoculation of different species on cowpea nodulation and soil fertility in Oyo town, Nigeria. Three Rhizobium spp. were isolated from the root nodules of Arachis hypogaea (BMP_MO, BMP_MA, and BMP_MK). A cowpea breeding line (IT95K-207-22) was grown in sterilized soil; the plants were inoculated with three Rhizobium species, a combination of two species, and a non-inoculated control for 6 weeks in a pot experiment. The experiment followed a 1 × 5 factorial in a completely randomized design with two replications. Data on nodule number, weight, colour, and soil fertility were collected and analyzed. Inoculation with Rhizobium species resulted in nodule formation on the roots of all three species and the consortium. Plants inoculated with BMP_MN_24 produced the significantly highest nodule weight (g) (0.3375), followed by BMP_MO_24 (0.2733), and the control (0.1524). However, BMP_MK_24 had the lowest nodule weight (0.0284). BMP_MK_24 was identified as Rhizobium leguminosarum (PX671459), BMP_MN_24 as Rhizobium sophorae (PX583111.1), and BMP_MO_24 as Rhizobium leguminosarum (PX583112.1). Rhizobium leguminosarum (BMP_MK_24) improved soil conditions, while Rhizobium sophorae (BMP_MN_24) enhanced cowpea nodulation more effectively than the other inoculants studied. These strains can be utilized as potential bio-fertilizers.