Browsing by Author "Samson, Oyindamola John"
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Item Biodegradative Capability of Molecularly Identified Pseudomonas fluorescens Strains on Palm Oil-Polluted Soil(LYAS, 2026-05-24) Popoola, Bukola Margaret; Afelumo, Ayooluwa; Samson, Oyindamola JohnOil palm (Elaeis guineensis) is an important source of edible vegetable oil derived from the fruit mesocarp; however, oil extraction commonly generates wastewater and residues that may be discharged into the environment without adequate treatment. Because microorganisms can degrade organic contaminants in palm oil mill effluent and oil-contaminated matrices, this study aimed to evaluate the potential of Pseudomonas fluorescens for remediating palm oil-contaminated soil. Palm oil and P. fluorescens isolates were obtained from Fiditi Oil Mill and Ajayi Crowther University, respectively, in Oyo State, Nigeria. Uncontaminated soil, oil mill soil, and purposefully contaminated soil were used as experimental control samples, while four purposefully contaminated soil samples and four oil mill soil samples were treated with P. fluorescens under varying soil sterilization and tillage conditions. The isolate was identified using 16S rRNA gene sequence analysis, and a 12-week field bioremediation trial was conducted to assess microbial load and residual oil content. The 16S rRNA sequencing confirmed the isolate as P. fluorescens MN607218.1. Residual oil in tilled sterile purposefully contaminated soil decreased from 0.13 g on day 0 to 0.10 g by week 12, whereas the corresponding non-sterile soil showed a greater reduction to 0.07 g by week 12. These findings indicate that P. fluorescens can effectively degrade palm oil in contaminated soil, particularly under non-sterile conditions, and may serve as a promising microbial agent for environmental cleanup of palm oil-contaminated sites.Item Impact of Rhizobium spp. on nodulation of cultivated (Vigna unguiculata) cowpea and soil fertility in southwestern Nigeria(Springer Nature, 2026-01-12) Popoola, Bukola Margaret; Oyatokun, Olukayode Stephen; Ezeoma, Chinenyenwa Mercy; Samson, Oyindamola JohnCowpea, an essential legume in the semi-arid regions of Sub-Saharan Africa, often experiences low yields due to poor cultivars, inadequate management practices, and limited agricultural inputs. This study evaluated the impact of Rhizobium inoculation of different species on cowpea nodulation and soil fertility in Oyo town, Nigeria. Three Rhizobium spp. were isolated from the root nodules of Arachis hypogaea (BMP_MO, BMP_MA, and BMP_MK). A cowpea breeding line (IT95K-207-22) was grown in sterilized soil; the plants were inoculated with three Rhizobium species, a combination of two species, and a non-inoculated control for 6 weeks in a pot experiment. The experiment followed a 1 × 5 factorial in a completely randomized design with two replications. Data on nodule number, weight, colour, and soil fertility were collected and analyzed. Inoculation with Rhizobium species resulted in nodule formation on the roots of all three species and the consortium. Plants inoculated with BMP_MN_24 produced the significantly highest nodule weight (g) (0.3375), followed by BMP_MO_24 (0.2733), and the control (0.1524). However, BMP_MK_24 had the lowest nodule weight (0.0284). BMP_MK_24 was identified as Rhizobium leguminosarum (PX671459), BMP_MN_24 as Rhizobium sophorae (PX583111.1), and BMP_MO_24 as Rhizobium leguminosarum (PX583112.1). Rhizobium leguminosarum (BMP_MK_24) improved soil conditions, while Rhizobium sophorae (BMP_MN_24) enhanced cowpea nodulation more effectively than the other inoculants studied. These strains can be utilized as potential bio-fertilizers.