Assessment of the toxicity and biochemical effects of detergent processed cassava on renal function of Wistar rats

Abstract

Fufu is a major component of food in West Africa. Local cassava farmers use detergent to ferment cassava root tuber during processing into fufu. Studies have established the hazardous health effects of detergent in humans. This study was carried out to determine the median lethal toxicity of detergent-processed cassava, and assessed its effects on the renal function of male Wistar rats. Fufu with different concentrations of detergent (250, 500 and 750 g/l) were given as food twice daily to the rats for the acute toxicity study. The sub-chronic test lasted for four weeks, during which the fufu samples prepared with 10, 20, 30, 40 and 50 g/l of detergent, were separately given to five groups of five rats, respectively. The results of the acute toxicity test showed increase in mortality with corresponding increase in detergent concentration. Plasma and urine creatinine and urea concentrations of all the detergent-treated rats were significantly higher than that of the negative and positive controls. Urinalysis showed significant presence of glucose and ketone bodies, as well as, blood and protein. Renal photomicrographs showed deranged cortical structure with diffuse loss of tubules, inflammatory cells infiltrate in the interstitium. The study concluded that detergent-processed cassava has adverse effects on the structure and function of the kidneys of rats.