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Browsing Faculty of Natural Sciences by Author "Adebami, Gboyega Ebenezer"
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Item PHYSICOCHEMICAL AND MICROBIAL EVALUATIONS OF DIFFERENT FISH PONDS’ WASTEWATERS AND THE ANTIBIOTICS PROFILES OF ISOLATED BACTERIA(Ethiopian Journal of Environmental Studies & Management, 2020-08-06) Adebami, Gboyega Ebenezer; Fasiku, Samuel Adedayo; Solomon, O D; Babalola B AAquaculture is a global practice that entails the breeding of different fish species. It is incessant in the Nigerian community as wastewater released from pond farms into surrounding drainages and water bodies cause pollution. In this study, a comparative analysis of the physico-chemical parameters (temperature, pH, salinity, conductivity, Dissolved Oxygen (DO) and Biological Oxygen Demand (BOD), and microbial evaluation of wastewater samples from concrete, plastic, wooden and earthen fish ponds located in Ogun and Lagos states followed with antibiotics sensitivity profile of the isolates were investigated. The values of temperature, pH, salinity, conductivity, DO and BOD of the wastewaters ranged from 23.83 – 24.67 °C, 6.57 – 9.68, 0.08 – 0.26 ppt, 65.67 – 200.7 µS/cm, 0.67 – 1.63 mg/L and 36.67 – 253.3 mg/L, respectively. Aerobic plate count and coliform count of bacteria isolated from different fish ponds wastewaters ranged from 6.40x105 to 4.20x106 cfu/mL and 2.80x103 to 1.98x105 cfu/mL, respectively. Enterococcus spp., Staphylococcus spp, Streptococcus spp., and Micrococcus spp., Aeromonas spp., Chromobacterium spp., Escherichia spp., Klebsiella spp. Listeria spp., Pseudomonas spp., Proteus spp., Providencia sp., Shigella sp., and Salmonella spp. were isolated from different ponds wastewaters. All selected bacterial isolates were susceptible to ciprofloxacin with the exception of Streptococcus sp., Chromobacterium sp. and Providencia sp. Antibiotics susceptibility pattern of isolated bacteria showed that most of the isolates had very high rate of resistance to the common antibiotics which could pose danger to the environment. The results obtained from this study suggests that control of wastewater release from fishpond farms to environment is necessary in other to maintain a stable environment, thereby reducing pollution.Item The decolourising potential of some laccase-producing Curvularia and Paradictyoarthrinium species(Discovery, 2026-03-07) Fasiku, Samuel Adedayo; Akinrinsade, Godwin Adeoluwa; Aregbesola, Seun Angel; Olajide, Benedicta Oyindamola; Afolabi, Femi Johnson; Adebami, Gboyega EbenezerLaccases are enzymes that break down lignin and are also useful in dye decolourisation. This study investigated laccase production by some laccase-producing fungi and evaluated their ability to decolourise dyes. Some fungi with laccase-producing potential were collected and identified using molecular techniques. They were cultivated on various substrates at pH 5 and 6. Laccase production was determined using 2, 2'-azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) [ABTS] and guaiacol as indicators. The fungi were grown in fermentation media containing 1 g/L of dyes, and their decolourising capacity was determined. The fungi were identified as Paradictyoarthrinium diffractum AP3, Paradictyoarthrinium diffractum AP2, Curvularia lunata DP1, Curvularia lunata FESAMB, Curvularia petersonii K2, and Curvularia verruculosa K6. There was higher laccase activity in substrates containing lignin than in those without. The highest laccase production (39.62 U/mL) was recorded by Curvularia lunata DPI in maize straw-containing medium at pH 6 with ABTS as an indicator. In comparison, Curvularia petersonii K2 was the best producer of laccase (17.67 U/mL) when guaiacol was used. Paradictyoarthrinium diffractum AP2 decolourised methyl red, Congo red and safranine, whereas Curvularia lunata FESAMB achieved the highest decolourisation rate (63%) of Leishmann blue. Curvularia and Paradictyoarthrinium species used in this work produced laccase enzymes, and successfully decolourised dyes.